Comparisons of microRNA microarray platforms and normalization methods

University of South Alabama

Wednesday, September 30, 2009 - 3:30pm

Microarray technology has been used to measure the messenger RNA (mRNA) expression in gene expression profiling studies. In recent years, this technology has been applied in microRNA (miRNA) discovery. In this study, we profile miRNAs from a panel of osteosarcoma xenografts individually using LNA microarray, beads-based array and TLDA cards, and compare the consistency of these three platforms. Several new miRNA normalization methods will also be investigated, and their performance will be evaluated by comparing with some existing normalization methods.